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Tuesday, May 29th, 2012

_scientists_ [ naufiel ]

11:15a Paper Request I realize Wiley has weird access permissions, but if anyone can get a pdf of this review for me, I would be enormously grateful. Wiley Interdiscip Rev Syst Biol Med. 2010 Sep-Oct;2(5):550-65. Systems analysis of alternative splicing and its regulation. Xiao X, Lee JH. email delinka at gmail dot com Thanks so much!

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Saturday, May 26th, 2012

_scientists_ [ oystermato ]

4:28p Paper Requests I need access to: NEUROCRITICAL CARE Volume 10, Number 3 (2009), 295-305, DOI: 10.1007/s12028-008-9177-5 Cerebrovascular Complications of Methamphetamine AbuseEmily L. Ho, S. Andrew Josephson, Han S. Lee and Wade S. Smith Am J Forensic Med Pathol. 2004 Dec;25(4):334-7. Spontaneous intracerebral hemorrhage related to methamphetamine abuse: autopsy findings and clinical correlation. McGee SM, McGee DN, McGee MB. Thanks! Edit: Thanks to the two very kind people who sent them to me!

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Friday, May 25th, 2012

lolscience [ beccastareyes ]

10:14a Spacelol The ISS crew grabs the Dragon space capsule, making it the first commercial vehicle to be docked to the ISS. Here's Dragon and the ISS's robotic arm. (Well, getting it berthed is in progress as I type...)

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Thursday, May 24th, 2012

lab_gripes [ dune_drd ]

2:11a Help me lab gripes, you're my only hope So I'm doing long-term fluorescent studies on Hela cells. Well, I used to do them in another lab, ever since we moved nothing is working anymore. Whenever I now try to image, my cells die. I tried everything; different cell batches, different buffers, gentler transfection methods, but nothing works. Whenever I set up the cells on the microscope, they bleb and die. And I mean all of them. And even if I manage to image some before they die, that's really not the point of long-term measuring, you know? I am completely clueless, and the boss is getting impatient. I have installed a heating stage and use phenol-red RPMI supplemented with Glutamate and 15mM HEPES. I only remove FBS, which I don't want to interfere with my measurments. This has always worked before, a little starvation was always okay, but not anymore... any idea what could cause this? The cells look fine in the flask, but when I transfer them onto glass my problems start. :(

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Tuesday, May 22nd, 2012

lab_gripes [ small_chicken ]

8:53a Just an update OK, so I decided to play hardball: first author, or take it down. Well, I didn't put it quite that way in the email to the editor, but I did say that nothing except first author would be acceptable at this point, considering that I was kind enough to wait for her (rude) response before dashing off the emails to everybody and their grandmother. Secondly: the universsity has begun legal proceedings. I'm not sure what this means--I very much doubt that a court would be involved, though if it does and I end up testifying, it could get interesting. I wonder if she'll try to slander me in open court... Anyway, the moral of the story is, if you're trying to get back at someone in the sciences, plagarism is a really shitty way to go about it, especially since they could always have a backup on gmail.

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Sunday, May 20th, 2012

lab_gripes [ small_chicken ]

8:37p So I finally heard back from the editor (meaning that An finally got back to him, meaning that at least one other co-author has spoken with her about this, or else she's gotten wind of what I've done somehow and is desperate for damage control). An is willing to give me 4th author. On the one hand, I realize that this is a major improvement. On the other, well...as I said before, 85% of the text is mine. Take it or leave it?

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